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While the application of two MRM transitions is common practice for unit resolution HPLC-MS/MS systems, confirmation of detects by recording of HRMS fragments is not always implemented and may lead to low specificity (Das-Navarro et al

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In the OG, grade IV and V degenerations were predominantly concentrated from T11/12 to L2/3, whereas in the CG, they were mainly at L4/5 and L5/S1 levels