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Trindade, F

Diabetic nephropathy: update on pillars of therapy slowing progression

Total protein from freshly brushed and cultured HAECs was collected in cell lysis buffer, homogenized by sonication, and then centrifuged at 1,699 g for 10 minutes

Jung et al., 2021)

Based on the amount of GSH in the lysate, we calculated the GSH concentration assuming an averaged HeLa/HT1080 cell volume of 4,000 m 3 (refs 15, 51, 52)